DYKDDDDK-Tag (FLAG-tag) rabbit pAb
- 公司名稱 上海貝博生物科技有限公司
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- 廠商性質 生產廠家
- 更新時間 2025/7/10 18:51:53
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Source
Rabbit
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Applications
WB,ELISA
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Reactivity
Species independent
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Dilution
WB: 1:1000 - 3000; ELISA: 1:20000. Not yet tested in other applications.
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Storage
-20°C/1 year
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Specificity
FLAG-tag Polyclonal Antibody detects FLAG-tagged recombinant proteins or FLAG-tagged proteins overexpressed in cells.
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Source/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Immunogen
DDDDK synthetic peptide conjugated to KLH.
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Form
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.05% sodium azide.
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Clonality
Polyclonal
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Background
The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.
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Concentration
1 mg/ml
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