基底膜剛度細(xì)胞牽張培育裝置系統(tǒng)，基底膜彈性可調(diào)細(xì)胞牽張拉伸動(dòng)態(tài)培養(yǎng)或靜態(tài)培養(yǎng)（1-180kpa）,適合原代和干細(xì)胞牽張拉伸培養(yǎng)，BioFlex CellSoft培養(yǎng)板是當(dāng)今wei一可在商業(yè)上可行的可施加細(xì)胞拉伸負(fù)荷的柔軟表面。

美國(guó)Flexcell細(xì)胞應(yīng)力加載培養(yǎng)與實(shí)時(shí)觀察分析系統(tǒng)，包括：
1.1.FX-5000T體外細(xì)胞拉應(yīng)力加載培養(yǎng)與實(shí)時(shí)觀察分析系統(tǒng)
1.2.FX-5000C細(xì)胞組織壓應(yīng)力加載培養(yǎng)與實(shí)時(shí)觀察分析系統(tǒng)
1.3.FX-5000TT細(xì)胞組織三維拉應(yīng)力加載培養(yǎng)與實(shí)時(shí)觀察分析系統(tǒng)
1.4.STR-4000流體剪切應(yīng)力加載培養(yǎng)與實(shí)時(shí)觀察分析系統(tǒng)。

其產(chǎn)品成熟度高、成功應(yīng)用文獻(xiàn)量達(dá)4000多篇，國(guó)內(nèi)有包括上海交通大學(xué)、復(fù)旦大學(xué)、同濟(jì)大學(xué)、上海第九醫(yī)院、中科院力學(xué)所、北京大學(xué)第三醫(yī)院、北航生物與醫(yī)學(xué)工程學(xué)院、都醫(yī)科大學(xué)、廣州醫(yī)科大學(xué)、南方科技大學(xué)、福建協(xié)和醫(yī)院、南方醫(yī)科大學(xué)近100家成功高校、醫(yī)院及基礎(chǔ)科研單位使用，無技術(shù)風(fēng)險(xiǎn)和使用風(fēng)險(xiǎn)，flexcell體外高通量細(xì)胞牽張拉伸力、壓應(yīng)力以及流體剪切力加載培養(yǎng)系統(tǒng)已成為細(xì)胞力學(xué)體外加載模型的黃金標(biāo)準(zhǔn)，是細(xì)胞組織力學(xué)研究者的shou選。

CellSoft™ Soft Substrate References

Key References:

Buxboim et al., How deeply do cells feel:methods for thin gels. J Phys Condens matter. 22: 1-19, 2010.

‍

Chodhury et al., Soft substrates promote homogeneous self-renewal of embryonic stem cells via down regulating cell-matrix tractions. PLOS 1 5: 1-10, 2010.

‍

Engle et al., Matrix elasticity directs stem cell lineage specification. Cell 126: 677-689, 2006.

‍

Hirata and Yamaoka. Effect of stem cell niche elasticity/ECM protein on the self-beating cardiomyocyte differentiation of iPS cells at different stages. Acta Biomat. 65: 44-52, 2018

‍

Megone et al., Impact of surface adhesion and sample heterogeneity on the multiscale mechanical characterization of soft biomaterials. Sci Rep 8: 6780, 2018.

‍

Ou et al., Visualizing mechanical modulation of nanoscale organization of cell-matrix adhesions. Integr. Biol. 8: 795-804,2016.

‍

Palchesko et al., Development of polydimethylsiloxane substrates with tunable elastic modulus to study cell mechanobiology in muscle and nerve. PLOS 1 7: 1-13, 2012

‍

Smith et al., Mechanosensing of matrix by stem cells: from matrix heterogeneity, contractility and the nucleus in pore-migration to cardiogenesis and muscle stem cells in vivo. Sem Cell Devtbio. 71: 84-98, 2017

‍

Solon et al., Fibroblast adaptation andstiffness matching to soft elastic substrates. Biophys J. 93: 4453-4461, 2007.

‍

Thomas et al., Measuring the mechanical properties of living cells using atomic force microscopy. J Visual Exp. 76:1-8, 2013.

‍

Vertelov et al., Rigidity of silicone substrates controls cell spreading and stem cell differentiation. Sci Rep. 6:1-10, 2016.